A simple, sensitive, precise and specific reverse phase high performance liquid chromatographic method was developed and validated for the simultaneous determination of aspirin and prasugrel hydrochloride in pharmaceutical dosage form. It was found that the excipient in the tablet dosage form does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography on Qualisil Gold C8 (5 μm; 250 x 4.6 mm) with a mobile phase composed of 5 mM Phosphate buffer (pH-3.0):Methanol (20:80 v/v) in isocratic mode at a flow rate of 1 ml/min. The detection was monitored at 238 nm. The calibration curve for aspirin and prasugrel HCl was linear from 2 to 18 and 15 to 135 µg/ml. The interday and intraday precision was found to be within limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of aspirin and prasugrel HCl in bulk and its tablet dosage forms. LOD and LOQ for aspirin were found to be 10 ng/ml and 30 ng/ml and prasugrel HCl were found to be 30 ng/ml and 100 ng/ml respectively. Accuracy and reproducibility were found satisfactory.
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